Dubremetz J F, Elsner Y Y
J Protozool. 1979 Aug;26(3):367-76. doi: 10.1111/j.1550-7408.1979.tb04639.x.
First-generation schizogony of Eimeria bovis in bovine cell culture was studied by electron microscopy. The intracellular sporozoite retained its structure for at least 6 days at which time it rounded up and lost its apical complex. Although the refractile body underwent certain morphologic changes, it was retained throughout the parasite's growth. The beginning of mitosis was marked by the formation of a cytoplasmic funnel which traversed the nucleus opening on each side toward a pair of centrioles. Subsequently, there developed an intranuclear spindle. Separation of the daughter nuclei was preceded by the formation of typical centrocones. Differentiation of merozoites was accomplished by exogenesis during the last mitotoc division. A dense fiber, interpreted as a link connecting the merozoite anlage with its nucleus, extended from the developing apical complex to the nearest division pole. In the anlage, the inner membrane complex was at first composed of patches associated with pairs of subpellicular microtubules. Rhoptries appeared early in merogenesis, whereas micronemes formed at the time the merozoites detached from the residuum. The level of amylopectin, low in schizonts, rose at the beginning of merozoite formation.
通过电子显微镜研究了牛艾美耳球虫在牛细胞培养物中的第一代裂殖生殖。细胞内子孢子至少6天保持其结构，此时它变圆并失去其顶端复合体。尽管折光体经历了某些形态变化，但在整个寄生虫生长过程中它都保留了下来。有丝分裂开始的标志是形成一个细胞质漏斗，该漏斗穿过细胞核，在两侧朝向一对中心粒开口。随后，形成了核内纺锤体。典型中心锥的形成先于子核的分离。裂殖子的分化在最后一次有丝分裂期间通过外生作用完成。一条致密纤维，被解释为连接裂殖子原基与其细胞核的纽带，从发育中的顶端复合体延伸到最近的分裂极。在原基中，内膜复合体最初由与成对的表膜下微管相关的斑块组成。棒状体在裂殖生殖早期出现，而微线体在裂殖子从残体分离时形成。裂殖体中支链淀粉水平较低，在裂殖子形成开始时升高。
