Evans D A
J Protozool. 1979 Aug;26(3):425-7. doi: 10.1111/j.1550-7408.1979.tb04648.x.
Culture procyclic forms of Trypanosoma brucei rhodesiense and Trypanosoma congolense were fed to Glossina morsitans morsitans through artificial membranes. A very high percentage of the flies so fed produced established midgut infections, a proportion of which went on to develop into mature metacyclic trypanosomes capable of infecting mammalian hosts. The method offers a safe, clean way of infecting tsetse flies with African trypanosomes which reduces the need for trypanosome-infected animals in the laboratory.
通过人工膜将布氏罗得西亚锥虫和刚果锥虫的前循环型培养物投喂给采采蝇。用这种方式投喂的采采蝇中有很高比例产生了肠道感染，其中一部分继续发育成能够感染哺乳动物宿主的成熟后循环型锥虫。该方法提供了一种安全、清洁的方式来用非洲锥虫感染采采蝇，减少了实验室中对感染锥虫动物的需求。
