Minter-Goedbloed E, Pudney M, Kilgour V, Evans D A
Z Parasitenkd. 1983;69(1):17-26. doi: 10.1007/BF00934006.
Trypanosomes, isolated from the gut of a naturally infected Glossina pallidipes in Kiboko, Kenya, were grown in vitro. The cultured trypanosomes ("F4"-stock) showed a wide variety in morphological stages, not characteristic of the salivarian trypanosomes that are known to occur in the Kiboko area. Identification of the "F4"-stock was attempted by isoenzyme studies, infection of tsetse flies and of experimental animals. Electrophoretic isoenzyme patterns of the "F4"-organisms were developed for ten soluble enzymes and compared with those of 5 reference stocks of salivarian trypanosomes and 2 stocks of Trypanosoma theileri. For the T. theileri comparisons six enzymes were used. It was found that the "F4"-trypanosome clearly differed from the 3 salivarian species and from T. theileri. Experimental infection of laboratory bred Glossina m. morsitans showed that the "F4"-organisms developed in the posterior station of the alimentary tract and the cultured trypanosomes were infective to non-indigenous reptiles. This is believed to be the first record of the isolation and the isoenzyme characterisation of a non-salivarian reptile trypanosome from a naturally infected tsetse fly of the morsitans group (subgenus Glossina) in a savannah area in Africa.
从肯尼亚基博科一只自然感染的淡足舌蝇肠道中分离出的锥虫，在体外进行培养。培养的锥虫（“F4”株系）在形态阶段表现出广泛的多样性，这并非已知在基博科地区出现的涎源性锥虫的特征。通过同工酶研究、感染采采蝇和实验动物来尝试鉴定“F4”株系。针对十种可溶性酶绘制了“F4”生物体的电泳同工酶图谱，并与5个涎源性锥虫参考株系以及2个泰勒锥虫株系的图谱进行比较。对于泰勒锥虫的比较使用了六种酶。结果发现，“F4”锥虫与3种涎源性锥虫物种以及泰勒锥虫明显不同。对实验室饲养的冈比亚采采蝇进行实验感染表明，“F4”生物体在消化道的后部发育，并且培养的锥虫对非本地爬行动物具有感染性。据信，这是首次从非洲稀树草原地区一只自然感染的采采蝇（Glossina亚属，采采蝇组）中分离出非涎源性爬行动物锥虫并对其进行同工酶特征描述的记录。
