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用于检测鼠疫耶尔森氏菌色素沉着的刚果红琼脂平板培养基。

Congo red-agar plating medium for detecting pigmentation in Pasteurella pestis.

作者信息

Surgalla M J, Beesley E D

出版信息

Appl Microbiol. 1969 Nov;18(5):834-7. doi: 10.1128/am.18.5.834-837.1969.

DOI:10.1128/am.18.5.834-837.1969
PMID:5370459
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC378096/
Abstract

Ability to detect pigmented and nonpigmented Pasteurella pestis is essential in plague research, and is currently dependent on use of the synthetic hemin-agar of Jackson and Burrows. We have devised a new differential medium for this purpose, containing Congo red dye and common, commercially available laboratory media. The ease and simplicity of preparation make the Congo red-agar a practical routine laboratory tool in plague research. These findings, possibly indicating a common binding site for hematin and Congo red, should be useful in efforts to determine the chemical nature of a bacterial component associated with high virulence in P. pestis.

摘要

在鼠疫研究中，检测色素沉着和无色素沉着的鼠疫耶尔森氏菌的能力至关重要，目前这依赖于使用杰克逊和伯罗斯的合成血红素琼脂。我们为此设计了一种新的鉴别培养基，它含有刚果红染料和常见的市售实验室培养基。制备的简便性使刚果红琼脂成为鼠疫研究中实用的常规实验室工具。这些发现可能表明血红素和刚果红有一个共同的结合位点，这对于确定鼠疫耶尔森氏菌中与高毒力相关的细菌成分的化学性质的研究应是有用的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc6a/378096/6e7573899744/applmicro00011-0149-e.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc6a/378096/6e7573899744/applmicro00011-0149-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc6a/378096/ef4e7e7828f2/applmicro00011-0149-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc6a/378096/ea53ce53cde1/applmicro00011-0149-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc6a/378096/c2e7acbde7f1/applmicro00011-0149-c.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc6a/378096/6e7573899744/applmicro00011-0149-e.jpg

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AZOTOBACTER VINELANDII RIBONUCLEIC ACID POLYMERASE. I. INHIBITION BY CONGO RED.维氏固氮菌核糖核酸聚合酶。I. 刚果红的抑制作用
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VIRULENCE OF PASTEURELLA PESTIS AND IMMUNITY TO PLAGUE.鼠疫杆菌的毒力与鼠疫免疫
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[IMMUNITY IN PLAGUE. ACQUISITIONS SUPPLIED BY 30 YEARS OF WORK ON THE "PASTEURELLA PESTIS EV" (GIRARD AND ROBIC) STRAIN].[鼠疫中的免疫。基于“鼠疫耶尔森氏菌EV”（吉拉尔和罗比克）菌株30年研究工作所取得的成果]
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Appl Environ Microbiol. 2023 Feb 28;89(2):e0124422. doi: 10.1128/aem.01244-22. Epub 2023 Feb 6.
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Protection Induced by Oral Vaccination with a Recombinant Delivering LcrV and F1 Antigens in Mice and Rats against Pneumonic Plague.口服接种携带 LcrV 和 F1 抗原的重组 对小鼠和大鼠肺鼠疫的保护作用。
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Deletion of Yersinia pestis Causes Temperature-Sensitive Pleiotropic Effects, Including Cell Lysis, That Are Suppressed by Carbon Source, Cations, or Loss of Phospholipase A Activity.鼠疫耶尔森氏菌缺失导致温度敏感的多效性效应，包括细胞裂解，这些效应受到碳源、阳离子或磷脂酶 A 活性丧失的抑制。
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