Kaji A, Ichimi T
Appl Microbiol. 1969 Dec;18(6):1036-40. doi: 10.1128/am.18.6.1036-1040.1969.
When Corticum rolfsii was grown in a medium containing bran extract under aerobic conditions, it secreted alpha-D-galactosidase and beta-D-galactosidase into the culture fluid. Pectin also stimulated the production of these enzymes, whereas galactose, glucose, and sucrose stimulated their production to a lesser degree. C. rolfsii produced greater amounts of both enzymes than Aspergillus niger. Both galactosidases in the culture medium hydrolyzed alpha- and beta-p-nitrophenyl-D-galactosides as well as lactose, stachyose, melibiose, and raffinose. Both exhibited optimal activity at pH 2 to 4 and were quite stable under acidic conditions. alpha-Galactosidase was separated from beta-galactosidase by column chromatography.
当罗氏白绢菌在含有麸皮提取物的培养基中好氧培养时，它会向培养液中分泌α-D-半乳糖苷酶和β-D-半乳糖苷酶。果胶也会刺激这些酶的产生，而半乳糖、葡萄糖和蔗糖对它们产生的刺激程度较小。罗氏白绢菌产生的这两种酶的量都比黑曲霉多。培养基中的两种半乳糖苷酶都能水解α-和β-对硝基苯基-D-半乳糖苷以及乳糖、水苏糖、蜜二糖和棉子糖。两者在pH 2至4时表现出最佳活性，并且在酸性条件下相当稳定。通过柱色谱法将α-半乳糖苷酶与β-半乳糖苷酶分离。
