某些放线菌目菌株的α-半乳糖苷酶活性调查。
Survey of some Actinomycetales for alpha-galactosidase activity.
作者信息
Lyons A J, Pridham T G, Hesseltine C W
出版信息
Appl Microbiol. 1969 Oct;18(4):579-83. doi: 10.1128/am.18.4.579-583.1969.
The enzyme alpha-galactosidase offers potential to (i) eliminate possibly the flatus-inducing factor(s) in edible beans, (ii) eliminate raffinose during beet-sugar processing, and (iii) determine raffinose analytically. Accordingly, 20 genera of the order Actinomycetales Buchanan 1917 were tested for evidence of alpha-galactosidase activity. Test filtrates were prepared with a medium containing D-galactose and soybean meal. Enzyme activity was demonstrated through cellulose thin-layer chromatography. Of 123 strains tested, 28 produced extracellular alpha-galactosidase. Almost all were streptomycetes. Members of the genera Actinoplanes Couch 1950, Micromonospora varphiOrskov 1923, and Promicromonospora Krasil'nikov et al. 1961 also exhibited alpha-galactosidase activity. Additional tests led to the selection of five strains whose filtrates degraded melibiose, raffinose, and stachyose but not lactose and sucrose. Tests also were made with several soybean preparations.
α-半乳糖苷酶这种酶有潜力：（i）消除食用豆类中可能导致肠胃胀气的因素；（ii）在甜菜制糖过程中消除棉子糖；（iii）用于棉子糖的分析测定。因此，对布坎南1917年放线菌目下的20个属进行了测试，以寻找α-半乳糖苷酶活性的证据。用含有D-半乳糖和豆粕的培养基制备测试滤液。通过纤维素薄层色谱法证明酶活性。在测试的123株菌株中，有28株产生细胞外α-半乳糖苷酶。几乎所有都是链霉菌。1950年考奇的游动放线菌属、1923年奥尔斯科夫的小单孢菌属以及1961年克拉西利尼科夫等人的原小单孢菌属的成员也表现出α-半乳糖苷酶活性。进一步的测试筛选出了五株菌株，其滤液能降解蜜二糖、棉子糖和水苏糖，但不能降解乳糖和蔗糖。还对几种大豆制品进行了测试。
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