Lohrmann R, Orgel L E
J Mol Evol. 1979 Dec;14(4):243-50. doi: 10.1007/BF01732491.
We have prepared substantial quantities of the alternating polymers poly(U-G) and poly(C-A) and have used them as templates for the self-condensation of ImpApC, ImpCpA, ImpGpU and ImpG. We find that the condensation of ImpGpU and ImpUpG on poly(C-A) is efficient, the condensation of ImpCpA on poly(U-G) is moderately efficient, while the condensation of ImpApC on poly(U-C) proceeds poorly. In many cases, the product is predominantly 3'-5'-linked. These reactions demonstrate unequivocally, for the first time, that template-directed reactions occur in double-helical structures. Furthermore, they describe for the first time a pair of reactions in which each of two complementary polymers facilitates the synthesis of the other. The prebiotic significance of these findings is discussed.
我们已经制备了大量的交替聚合物聚（U-G）和聚（C-A），并将它们用作ImpApC、ImpCpA、ImpGpU和ImpG自缩合的模板。我们发现，ImpGpU和ImpUpG在聚（C-A）上的缩合是有效的，ImpCpA在聚（U-G）上的缩合效率适中，而ImpApC在聚（U-C）上的缩合进行得很差。在许多情况下，产物主要是3'-5'-连接的。这些反应首次明确证明了模板导向反应发生在双螺旋结构中。此外，它们首次描述了一对反应，其中两种互补聚合物中的每一种都促进了另一种的合成。讨论了这些发现的益生元意义。
