Erdmann G, Wiegand H, Wellhöner H H
Naunyn Schmiedebergs Arch Pharmacol. 1975;290(4):357-73. doi: 10.1007/BF00499949.
The distribution of radioactivity in the sciatic nerve, the spinal ganglia, the ventral roots and the spinal cord was studied by means of histoautoradiography after injection of 125I-labelled tetanus toxin into gastrocnemius muscles of cats. In the sciatic nerve the major part of the radioactivity was found in the epineurium, but some axons also contained radioactivity. In the ventral root the radioactivity was strictly confined to a few axons; no radioactivity was found in other parts of the ventral root. In the spinal cord the radioactivity was confined to a few motoneurones where it was found in the soma as well as in the dendrites. Transient cooling of the ventral roots prevented the ascent of radioactivity into the spinal cord. Colchicine and vinblastine, after local application to the sciatic nerve, reduced the amount of radioactivity found in the ventral roots and in the spinal cord. However, the same effect was also obtained but to a lesser degree with lumicolchicine. It is concluded that the intraaxonal compartment is involved in the neural ascent of tetanus toxin into the spinal cord.
将125I标记的破伤风毒素注入猫的腓肠肌后，通过组织放射自显影术研究了放射性在坐骨神经、脊神经节、腹根和脊髓中的分布。在坐骨神经中，大部分放射性存在于神经外膜，但一些轴突也含有放射性。在腹根中，放射性严格局限于少数轴突；在腹根的其他部位未发现放射性。在脊髓中，放射性局限于少数运动神经元，在胞体和树突中均有发现。腹根的短暂冷却阻止了放射性向脊髓的上升。将秋水仙碱和长春碱局部应用于坐骨神经后，腹根和脊髓中发现的放射性量减少。然而，光秋水仙碱也有同样的效果，但程度较轻。得出的结论是，轴突内区室参与了破伤风毒素向脊髓的神经上行过程。
