破伤风毒素-[2,3-³H]丙酰胺。合成、比活性、免疫反应性、毒性及向脊髓的上行
Tetanus toxin-[2,3-3H]propionamid. Synthesis, specific activity, immunoreactivity, toxicity and ascent into the spinal cord.
作者信息
Meyer-Eppler T B, Wellhöner H H
出版信息
Arch Toxicol. 1983 Apr;52(4):303-10. doi: 10.1007/BF00316498.
PMID:6870543
Abstract
Tetanus toxin was tritiated with various amounts of N-succinimidyl-[2,3-3H]propionate. A tenfold molar excess of reagent over toxin was used for the introduction of about 5 nmol of [2,3-3H]propionate into 1 nmol of toxin, corresponding to a specific activity of about 48 MBq/mg protein. The labelling procedure did not change significantly the immunoreactivity of tetanus toxin, but reduced the toxicity in mice from 12 ng/kg to 16 ng/kg. Tetanus toxin-[2,3-3H]propionamid was carried through the peripheral nerves into the spinal cord. It could be used instead of 125I-labelled toxin for the detection of anti-tetanus toxin antibodies with a detection limit of 0.25 mU/ml of serum.
摘要
破伤风毒素用不同量的N-琥珀酰亚胺基-[2,3-³H]丙酸进行氚标记。试剂相对于毒素使用了10倍的摩尔过量，以便将约5 nmol的[2,3-³H]丙酸引入1 nmol的毒素中，这相当于约48 MBq/mg蛋白质的比活度。标记过程并未显著改变破伤风毒素的免疫反应性，但将其对小鼠的毒性从12 ng/kg降低至16 ng/kg。破伤风毒素-[2,3-³H]丙酰胺通过外周神经进入脊髓。它可替代¹²⁵I标记的毒素用于检测抗破伤风毒素抗体，血清检测限为0.25 mU/ml。
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Tetanus toxin-[2,3-3H]propionamid. Synthesis, specific activity, immunoreactivity, toxicity and ascent into the spinal cord.破伤风毒素-[2,3-³H]丙酰胺。合成、比活性、免疫反应性、毒性及向脊髓的上行
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