Roshchina V V, Ladygin V G
Prikl Biokhim Mikrobiol. 1979 Nov-Dec;15(6):883-8.
A method for qualitative determination of cytochromes of the wild-type and mutant strains of Chalmydomonas reinhardii was developed. The effect of different techniques of cell disruption (ultrasound, Triton X-100, acetone, etc) on detection of cytochrome maxima in the oxidized minus reduced difference spectra was studied on a comparative basis. The development of the stable difference spectrum of the disrupted cell suspension was shown to be a function of incubation time in the presence of an oxidizing or a reducing agent. Cytochromes of the wild-type and 10 nonphotosynthesizing mutants were determined. Four mutants with lesions in cytochromes B559 and C553 were detected. Mutants with lesions in the reaction center of photosystem 2 were found to have a substantially reduced content of cytochrome B559, whereas those with normal photosystems--of cytochrome C553.
开发了一种定性测定莱茵衣藻野生型和突变株细胞色素的方法。在比较的基础上，研究了不同细胞破碎技术（超声、Triton X-100、丙酮等）对氧化态减去还原态差光谱中细胞色素最大值检测的影响。结果表明，破碎细胞悬液稳定差光谱的形成是在氧化或还原剂存在下孵育时间的函数。测定了野生型和10个非光合突变体的细胞色素。检测到4个细胞色素B559和C553有损伤的突变体。发现光系统2反应中心有损伤的突变体中细胞色素B559的含量大幅降低，而光系统正常的突变体中细胞色素C553的含量则正常。
