菠菜叶绿体中的多聚半乳糖脂
Polygalactolipids in spinach chloroplasts.
作者信息
Webster D E, Chang S B
出版信息
Plant Physiol. 1969 Nov;44(11):1523-7. doi: 10.1104/pp.44.11.1523.
Two polygalactolipids, designated as components A and B, were isolated from spinach chloroplasts and were also obtained from glycolipid products synthesized with chloroplast enzymes using uridine diphosphate galactose as a galactose donor. These lipids were purified by column and thin layer chromatography. Chemical analysis of component A indicates that the lipid is trigalactosyl diglyceride, whereas component B behaves like tetragalactosyl diglyceride on a thin layer plate. The major fatty acid in trigalactosyl diglyceride was alpha-linolenic acid. Relative amount (molar ratio) of galactolipids in spinach chloroplasts was monogalactosyl diglyceride:digalactosyl diglyceride:trigalactosyl diglyceride:(tetragalactosyl diglyceride) = 60:30:5:1.
从菠菜叶绿体中分离出两种多聚半乳糖脂，分别命名为组分A和组分B，它们也可从以尿苷二磷酸半乳糖作为半乳糖供体、用叶绿体酶合成的糖脂产物中获得。这些脂质通过柱色谱和薄层色谱进行纯化。组分A的化学分析表明该脂质是三半乳糖基二甘油酯，而组分B在薄层板上的行为类似于四半乳糖基二甘油酯。三半乳糖基二甘油酯中的主要脂肪酸是α-亚麻酸。菠菜叶绿体中半乳糖脂的相对含量（摩尔比）为单半乳糖基二甘油酯：二半乳糖基二甘油酯：三半乳糖基二甘油酯：（四半乳糖基二甘油酯）=60:30:5:1。
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