盘基网柄菌中的三羧酸循环。代谢物浓度、氧气摄取及14C标记氨基酸标记模式。
The tricarboxylic acid cycle in Dictyostelium discoideum. Metabolite concentrations, oxygen uptake and 14c-labelled amino acid labelling patterns.
作者信息
Kelly P J, Kelleher J K, Wright B E
出版信息
Biochem J. 1979 Dec 15;184(3):581-8. doi: 10.1042/bj1840581.
Some aspects of tricarboxylic acid-cycle activity during differentiation and aging in Dictyostelium discoideum were examined. The concentrations of glutamate, aspartate, alanine, citrate, 2-oxoglutarate, succinate, fumarate, malate, oxaloacetate, pyruvate and acetyl-CoA were determined at four stages over the course of differentiation. The rate of O2 utilization was also determined over differentiation. In addition, experiments are described in which the specific radioactivities of citrate, 2-oxoglutarate, succinate, fumarate and malate were determined during a 30 min labelling of cells from the preculmination stage of development with [14C]glutamate, [14C]aspartate or [14C]alanine. A similar experiment was also performed with cells from the aggregation stage of development using [14C]glutamate.
研究了盘基网柄菌在分化和衰老过程中三羧酸循环活性的某些方面。在分化过程的四个阶段测定了谷氨酸、天冬氨酸、丙氨酸、柠檬酸、2-氧代戊二酸、琥珀酸、富马酸、苹果酸、草酰乙酸、丙酮酸和乙酰辅酶A的浓度。还测定了分化过程中的氧气利用率。此外，还描述了一些实验，在这些实验中，用[14C]谷氨酸、[14C]天冬氨酸或[14C]丙氨酸对发育前期的细胞进行30分钟标记期间，测定了柠檬酸、2-氧代戊二酸、琥珀酸、富马酸和苹果酸的比放射性。使用[14C]谷氨酸对发育聚集期的细胞也进行了类似实验。
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