Myher J J, Kuksis A, Vasdev S C, Kako K J
Can J Biochem. 1979 Nov;57(11):1315-27. doi: 10.1139/o79-175.
Stereospecific degradation and combined gas chromatographic--mass spectrometric (gc/ms) analysis were employed in a detailed investigation of the triacylglycerol structure of mustard seed oil and of the triacylglycerols transiently accumulating in the hearts of young rats receiving the oil in their diet. It was shown that feeding of mustard seed oil at 40% of the daily caloric requirement resulted in a deposition of cardiac triacylglycerols containing a high proportion of enantiomers of a positional distribution and molecular association of fatty acids which were closely similar to those found in the dietary oil. Complete structures were derived for a total of 88 species representing 75 to 85% of the triacylglycerols. About 90% of the accumulated triacylglycerol contained at least one long-chain (C20--C22) monounsaturated fatty acid per molecule. The long-chain acids were confined mainly to the primary positions and preferentially to the sn-3-position of the glycerol molecule. The dietary lipidosis, is, therefore, accompanied by little or no accumulation of the normal rat tissue triacylglycerols containing C16 and C18 fatty acids. It is suggested that the deposition and eventual clearance of the enantiomeric long-chain triacylglycerols in the rat heart during mustard seed oil feeding may be largely a result of a gradual change in specificity of the cardiac lipases.
采用立体特异性降解和气相色谱 - 质谱联用（GC/MS）分析方法，对芥子油的三酰甘油结构以及饮食中摄入该油的幼鼠心脏中瞬时积累的三酰甘油进行了详细研究。结果表明，以每日热量需求的40%喂食芥子油，会导致心脏三酰甘油沉积，其中脂肪酸的位置分布和分子缔合的对映体比例很高，与食用油中的情况极为相似。共推导了88种三酰甘油的完整结构，占三酰甘油总量的75%至85%。积累的三酰甘油中约90%的分子每分子至少含有一种长链（C20 - C22）单不饱和脂肪酸。长链酸主要集中在甘油分子的伯位，优先位于sn - 3位。因此，饮食性脂质增多症伴随着正常大鼠组织中含C16和C18脂肪酸的三酰甘油很少或没有积累。有人认为，喂食芥子油期间大鼠心脏中对映体长链三酰甘油的沉积和最终清除，可能主要是心脏脂肪酶特异性逐渐变化的结果。
