针对烟草花叶病毒蛋白特定抗原决定簇的免疫反应研究。
Studies on the immune response to a characterized antigenic determinant of the tobacco mosaic virus protein.
作者信息
Spitler L, Benjamini E, Young J D, Kaplan H, Fudenberg H H
出版信息
J Exp Med. 1970 Jan 1;131(1):133-48. doi: 10.1084/jem.131.1.133.
THE FOLLOWING PEPTIDES HAVE PREVIOUSLY BEEN SHOWN TO BIND SPECIFICALLY WITH ANTIBODIES TO TMVP
(a) An eicosapeptide representing residues 93-112 of TMVP and having the sequence Ileu-Ileu-Glu-Val-Glu-AspNH(2)-GluNH(2)-Ala-AspNH(2)-Pro-Thr-Thr-Ala-Glu-Thr-Leu-Asp-Ala-Thr-Arg. (b) Its C-terminal decapeptide. (c) Its C-terminal pentapeptide. (d) N-octanoyl-C-terminal-tripeptide. (e) (Lys)(4)-C-terminal-pentapeptide. (f) (Lys)(7) C-terminal-pentapeptide. The present communication deals with the investigation of several parameters of the immunological activity of the peptides. The results show that none of the peptides tested were immunogenic in guinea pigs, nor did they stimulate the incorporation of (14)C-thymidine by spleen cells derived from TMVP-primed animals. Results also showed that all of the peptides tested could elicit specific delayed and immediate skin reactions in TMVP-sensitized guinea pigs, and furthermore, that the peptides could specifically inhibit the migration of peritoneal exudate cells derived from these animals. The elicitation of delayed skin reactions and the ability to inhibit migration of peritoneal exudate cells were independent of carrier specificity.
以下肽段先前已被证明能与抗TMVP抗体特异性结合：(a) 一种二十肽，代表TMVP的93 - 112位残基，序列为Ileu - Ileu - Glu - Val - Glu - AspNH(2) - GluNH(2) - Ala - AspNH(2) - Pro - Thr - Thr - Ala - Glu - Thr - Leu - Asp - Ala - Thr - Arg。(b) 其C末端十肽。(c) 其C末端五肽。(d) N - 辛酰基 - C末端三肽。(e) (Lys)(4) - C末端五肽。(f) (Lys)(7) C末端五肽。本通讯涉及对这些肽段免疫活性的几个参数的研究。结果表明，所测试的肽段在豚鼠中均无免疫原性，也未刺激来自经TMVP免疫的动物的脾细胞掺入(14)C - 胸腺嘧啶核苷。结果还表明，所有测试的肽段均可在TMVP致敏的豚鼠中引发特异性迟发型和速发型皮肤反应，此外，这些肽段可特异性抑制来自这些动物的腹腔渗出细胞的迁移。迟发型皮肤反应的引发以及抑制腹腔渗出细胞迁移的能力与载体特异性无关。
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