Snellen J E, Raj H D
J Bacteriol. 1970 Jan;101(1):240-9. doi: 10.1128/jb.101.1.240-249.1970.
Phenotypic expressions of morphogenesis and fine structure of Leucothrix mucor were determined when the organism was grown with and without added CaCl(2) in a synthetic seawater medium. Evidence is presented to show that a bulb can form in the absence of a knot formation and that a bulb may give rise to a "germ-tube." In comparison with normal cells, which show transverse septa at right angles to the axis in dividing cells, the bulbs exhibited transverse septa at odd angles, which may explain the mechanism of bulb formation. The most striking morphological feature due to Ca(++) deficiency was the absence of rosettes; instead, the culture showed an extremely filamentous morphology and a peculiar cord formation. Also, the Ca(++)-deficient cells contained heavily stained intracytoplasmic granules which possibly represent tight packing of the smaller particles of ribonucleoprotein. Various bulbous forms observed in the Ca(++)-deficient culture showed more pronounced elaboration of mesosomes as intracytoplasmic structures than those seen in the complete medium.
当在合成海水培养基中添加和不添加氯化钙培养粘液亮发菌时，对其形态发生的表型表达和精细结构进行了测定。有证据表明，在没有节形成的情况下可以形成球茎，并且球茎可能产生“芽管”。与正常细胞相比，正常细胞在分裂细胞中显示出与轴成直角的横向隔膜，而球茎则呈现出奇数角度的横向隔膜，这可能解释了球茎形成的机制。由于缺钙最显著的形态学特征是没有玫瑰花结；相反，培养物呈现出极其丝状的形态和特殊的索状结构。此外，缺钙细胞含有染色很深的胞质内颗粒，这些颗粒可能代表核糖核蛋白较小颗粒的紧密堆积。在缺钙培养物中观察到的各种球茎形式显示，作为胞质内结构的中介体比在完全培养基中观察到的更为明显。
