Lorković H, Hooker W M, Tomanek R J
Arch Int Physiol Biochim. 1975 Aug;83(3):493-504. doi: 10.3109/13813457509071394.
ACh contractures of denervated lumbricalis muscles of rats were washed in Tris-methanesulfonate solutions and in sucrose solutions. The peak tension was diminished by about 80% following a 30 min exposure to solutions containing 400-600 mM glycerol when Tris solutions were used in the testing period, and by about 50% when sucrose solutions were used. The amplitude of the membrane potential changes provoked by ACh was decreased by about 36% following the glycerol treatment. The treatment had no effect on the ACh-induced 45Ca uptake of muscles. Electron microscopy of the glycerol-treated muscles showed widespread vacuolization apparently originating from swelling and disruption of the T system. It was concluded that ACh receptors which arise in the muscle membrane following nerve section are distributed in the external surface membrane and in the membranes of the T system.
将大鼠失神经支配的蚓状肌的乙酰胆碱（ACh）挛缩物置于三（羟甲基）甲胺磺酸盐溶液和蔗糖溶液中冲洗。当在测试期使用三（羟甲基）甲胺溶液时，暴露于含有400 - 600 mM甘油的溶液30分钟后，峰值张力降低约80%；当使用蔗糖溶液时，峰值张力降低约50%。甘油处理后，ACh引发的膜电位变化幅度降低约36%。该处理对ACh诱导的肌肉45Ca摄取没有影响。对甘油处理的肌肉进行电子显微镜检查显示，广泛的空泡化显然源于T系统的肿胀和破坏。得出的结论是，神经切断后在肌膜中出现的ACh受体分布于外表面膜和T系统的膜中。
