A method for studying the peripheral mediators of the dental pain induced by electrical stimulation.

A useful method is described in this paper for studying the mediators released from tooth pulp of the dog during the electrical stimulation of dentine. This method is based upon the perfusion of the pulp and superfusing the return continuously over the isolated, in cascade, cat jejunum and rat stomach fundus strip. The presented evidences in this study indicate the possibility of the release of bradykinin and prostaglandin - like materials from the tooth pulp during the electrical stimulation of dentine. Possible relationship of these mediators and tooth pain due to the electrical stimulation of dentine is discussed.