毛霉和三孢布拉氏霉中的激素相互作用。
Hormonal interactions in Mucor mucedo and Blakeslea trispora.
作者信息
Van den Ende H, Wiechmann A H, Reyngoud D J, Hendriks T
出版信息
J Bacteriol. 1970 Feb;101(2):423-8. doi: 10.1128/jb.101.2.423-428.1970.
Evidence is presented that progametangia in both the plus and the minus mating types of Mucor mucedo can be induced by one substance, namely (-)-trisporic acid B. A method is described for the determination of the concentration of the sex factors (trisporone, trisporic acid B, trisporic acid C) in mated cultures of Mucorales by polarography. It can be demonstrated that the amount of plus mycelium is limiting for the production of the sex factors in Blakeslea trispora. It is shown that the minus type of this organism is able to synthesize the sex factors when incubated in the filtered medium of a mated culture. Cycloheximide and 5-fluorouracil inhibit strongly the sex factor production in a mated culture of B. trispora at any time. This result suggests that sexual activity comprises the synthesis of proteins which are involved in the production of the sex factors.
有证据表明，毛霉的正、负交配型中的原配子囊都可被一种物质即（-）-三孢酸B诱导。描述了一种通过极谱法测定毛霉目交配培养物中性别因子（三孢素、三孢酸B、三孢酸C）浓度的方法。可以证明，在三孢布拉氏霉中，正菌丝体的数量限制了性别因子的产生。结果表明，该生物体的负型在交配培养物的过滤培养基中培养时能够合成性别因子。放线菌酮和5-氟尿嘧啶在任何时候都强烈抑制三孢布拉氏霉交配培养物中性别因子的产生。这一结果表明，有性活动包括参与性别因子产生的蛋白质的合成。
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