反相液相色谱法鉴定大鼠组织中的4-O-甲基多巴胺（作者译）
[Identification of 4-O-methyldopamine in rat tissues by reverded-phase liquid chromatography (author's transl)].
作者信息
Bidard J N, Cronenberger L
出版信息
J Chromatogr. 1979 Oct 11;164(2):139-54.
4-O-Methyldopamine was identified and assayed in tissues from L-dopa treated rats by reversed-phase high-performance liquid chromatography. The initial steps in the separation of catecholamines were performed by alumine, a weak cation-exchange resin, and thin-layer chromatographic techniques. After L-[3 H] dopa administration, the radiochromatogram was superimposed on the fluorochromatogram obtained with authentic marker 4-O-methyldopamine. This metabolite was detected in kidney but not in brain. The 4-O-methyldopamine:3-O-methyldopamine ratio was 0.032 in kidney. The influence of various treatments on this ratio was investigated. A 160% increase was found after L-dopa administration. This effect was potentiated by nialamide pretreatment (550% increase).
采用反相高效液相色谱法对用左旋多巴治疗的大鼠组织中的4-O-甲基多巴胺进行了鉴定和测定。儿茶酚胺分离的初始步骤通过氧化铝（一种弱阳离子交换树脂）和薄层色谱技术进行。给予L-[3H]多巴后，将放射色谱图与用真实标记物4-O-甲基多巴胺获得的荧光色谱图叠加。在肾脏中检测到了这种代谢物，但在大脑中未检测到。肾脏中4-O-甲基多巴胺与3-O-甲基多巴胺的比率为0.032。研究了各种处理对该比率的影响。给予左旋多巴后发现增加了160%。尼亚酰胺预处理可增强这种作用（增加550%）。
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