Woestenborghs R, Michiels M, Heykants J
J Chromatogr. 1979 Oct 11;164(2):169-76. doi: 10.1016/s0378-4347(00)81185-8.
A method is described for the determination of the antiarrhythmic drug lorcainide hydrochloride and its three main metabolites in plasma, urine, faeces and tissues from man and animals. The procedure involves the extract of the parent drug, its metabolites and the internal standard from the biological materials at different alkaline pH values, back-extraction into sulphuric acid and re-extraction into the organic phase (heptane--isoamyl alcohol). After silylation of the different phenolic and the N-dealkylated metabolites, analyses were carried out by automated gas--liquid chromatography with electron-capture detection. The method has a sensitivity limit of 5 ng for lorcainide, and 10--20 ng for the various metabolites, per millilitre of plasma. The method was applied to urine, faeces, plasma and tissue samples from man and animals. It was also suitable for automatic sample analysis.
本文描述了一种测定人及动物血浆、尿液、粪便和组织中抗心律失常药物盐酸劳卡尼及其三种主要代谢物的方法。该方法包括在不同碱性pH值下从生物材料中提取母体药物、其代谢物和内标，反萃取到硫酸中，再萃取到有机相（庚烷 - 异戊醇）。对不同的酚类和N - 去烷基化代谢物进行硅烷化后，采用带电子捕获检测的自动气液色谱法进行分析。该方法对血浆中劳卡尼的检测限为每毫升5纳克，对各种代谢物的检测限为10 - 20纳克。该方法应用于人及动物的尿液、粪便、血浆和组织样本。它也适用于自动样品分析。
