Teale F W, Dale R E
Biochem J. 1970 Jan;116(2):161-9. doi: 10.1042/bj1160161.
Several phycobiliproteins were prepared chromatographically pure and their absorption, fluorescence-emission, fluorescence-excitation and fluorescence-excitation polarization spectra determined. Changes in these spectra with ionic strength of the aqueous medium and chromoprotein concentration were interpreted in terms of interchromophore energy transfer and protein subunit equilibria. The complexity of the polarization spectra confirms the presence of different types of chromophore, designated sensitizing (;s') and fluorescing (;f'), in a single protein.
通过色谱法制备了几种纯的藻胆蛋白,并测定了它们的吸收光谱、荧光发射光谱、荧光激发光谱和荧光激发偏振光谱。根据发色团间的能量转移和蛋白质亚基平衡,解释了这些光谱随水介质离子强度和色素蛋白浓度的变化。偏振光谱的复杂性证实了在单一蛋白质中存在不同类型的发色团,分别称为敏化发色团(s)和荧光发色团(f)。