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藻胆蛋白的分离与光谱表征

Isolation and spectral characterization of phycobiliproteins.

作者信息

Teale F W, Dale R E

出版信息

Biochem J. 1970 Jan;116(2):161-9. doi: 10.1042/bj1160161.

DOI:10.1042/bj1160161
PMID:5414092
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1185341/
Abstract

Several phycobiliproteins were prepared chromatographically pure and their absorption, fluorescence-emission, fluorescence-excitation and fluorescence-excitation polarization spectra determined. Changes in these spectra with ionic strength of the aqueous medium and chromoprotein concentration were interpreted in terms of interchromophore energy transfer and protein subunit equilibria. The complexity of the polarization spectra confirms the presence of different types of chromophore, designated sensitizing (;s') and fluorescing (;f'), in a single protein.

摘要

通过色谱法制备了几种纯的藻胆蛋白，并测定了它们的吸收光谱、荧光发射光谱、荧光激发光谱和荧光激发偏振光谱。根据发色团间的能量转移和蛋白质亚基平衡，解释了这些光谱随水介质离子强度和色素蛋白浓度的变化。偏振光谱的复杂性证实了在单一蛋白质中存在不同类型的发色团，分别称为敏化发色团（s）和荧光发色团（f）。

相似文献

1
Isolation and spectral characterization of phycobiliproteins.藻胆蛋白的分离与光谱表征
Biochem J. 1970 Jan;116(2):161-9. doi: 10.1042/bj1160161.
2
Fluorescence studies on R-phycoerythrin and C-phycoerythrin.藻红蛋白和藻蓝蛋白的荧光研究。
J Fluoresc. 1991 Jun;1(2):135-40. doi: 10.1007/BF00865209.
3
Low-temperature absorption and circular dichroism studies of phytochrome.植物色素的低温吸收与圆二色性研究
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4
Subunit structure of the phycobiliproteins of blue-green algae.蓝藻藻胆蛋白的亚基结构
Proc Natl Acad Sci U S A. 1971 Jul;68(7):1398-401. doi: 10.1073/pnas.68.7.1398.
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Characterization of phycoerythrin from a Cryptomonas sp.一种隐藻属藻类藻红蛋白的特性分析
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6
Phycobiliproteins.藻胆蛋白
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7
Deconvolution of C-phycocyanin beta-84 and beta-155 chromophore absorption and fluorescence spectra of cyanobacterium Mastigocladus laminosus.层理鞭枝藻中藻蓝蛋白β-84和β-155生色团吸收光谱与荧光光谱的去卷积分析
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[Chromophore composition and nature of the absorption spectra of phycobiliproteins].[藻胆蛋白的发色团组成及吸收光谱的性质]
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The native forms of the phycobilin chromophores of algal biliproteins. A clarification.藻类双蛋白藻胆素发色团的天然形式。一则说明。
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Photochemically active chromoprotein isolated from the blue-green alga Anabaena cylindrica.从蓝绿藻圆柱鱼腥藻中分离出的具有光化学活性的色蛋白。
Nature. 1968 Sep 21;219(5160):1270-1. doi: 10.1038/2191270a0.

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Direct single-molecule measurements of phycocyanobilin photophysics in monomeric C-phycocyanin.单体藻蓝蛋白中藻蓝胆素光物理的直接单分子测量。
Proc Natl Acad Sci U S A. 2017 Sep 12;114(37):9779-9784. doi: 10.1073/pnas.1705435114. Epub 2017 Aug 28.
5
Exciton interactions in phycoerythrin.藻红蛋白中的激子相互作用。
Photosynth Res. 1986 Jan;10(3):209-15. doi: 10.1007/BF00118285.
6
Fluorescence studies on R-phycoerythrin and C-phycoerythrin.藻红蛋白和藻蓝蛋白的荧光研究。
J Fluoresc. 1991 Jun;1(2):135-40. doi: 10.1007/BF00865209.
7
A novel staining protocol for multiparameter assessment of cell heterogeneity in Phormidium populations (cyanobacteria) employing fluorescent dyes.一种利用荧光染料对鱼腥藻属（蓝藻）进行多参数评估细胞异质性的新型染色方案。
PLoS One. 2013;8(2):e55283. doi: 10.1371/journal.pone.0055283. Epub 2013 Feb 20.
8
Flow of excitation energy in the cryptophyte light-harvesting antenna phycocyanin 645.隐藻光捕获天线藻蓝蛋白 645 中的激发能流。
Biophys J. 2011 Aug 17;101(4):1004-13. doi: 10.1016/j.bpj.2011.07.012.
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Phycoerythrins of the Red Alga Callithamnion: VARIATION IN PHYCOERYTHROBILIN AND PHYCOUROBILIN CONTENT.红藻 Callithamnion 的藻红蛋白：藻红胆素和藻蓝胆素含量的变化。
Plant Physiol. 1981 Aug;68(2):482-8. doi: 10.1104/pp.68.2.482.
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Biliproteins of cyanobacteria and Rhodophyta: Homologous family of photosynthetic accessory pigments.蓝细菌和红藻的藻胆蛋白：光合辅助色素的同源家族。
Proc Natl Acad Sci U S A. 1976 Feb;73(2):428-31. doi: 10.1073/pnas.73.2.428.

本文引用的文献

1
POLARIZATION OF FLUORESCENCE OF MUSCLE PROTEINS.肌肉蛋白质荧光的偏振
J Biol Chem. 1965 Jul;240:2888-99.
2
FLUORESCENCE POLARISATION AUD LOCATION OF FLUORESCENCE MAXIMA OF C-PHYCOCYANIN.藻蓝蛋白荧光最大值的荧光偏振及定位
Biochim Biophys Acta. 1965 Mar 29;94:579-81. doi: 10.1016/0926-6585(65)90068-3.
3
C-PHYCOCYANIN: MINIMUM MOLECULAR WEIGHT.C-藻蓝蛋白：最小分子量
Science. 1964 Sep 4;145(3636):1054-6. doi: 10.1126/science.145.3636.1054.
4
Protein chromatography on calcium phosphate columns.在磷酸钙柱上进行蛋白质色谱分析。
Arch Biochem Biophys. 1956 Nov;65(1):132-55. doi: 10.1016/0003-9861(56)90183-7.
5
Energy transfer between chromophore and protein in phycocyanin.藻蓝蛋白中发色团与蛋白质之间的能量转移
Arch Biochem Biophys. 1954 Mar;49(1):222-33. doi: 10.1016/0003-9861(54)90182-4.
6
Structure of phycoerythrobilin and phycocyanobilin.藻红胆素和藻蓝胆素的结构。
Nature. 1967 Sep 30;215(5109):1477-8. doi: 10.1038/2151477a0.
7
Distinct subunits of phycoerythrin from Porphyridium cruentum and their spectral characteristics.紫球藻藻红蛋白的不同亚基及其光谱特性。
Arch Biochem Biophys. 1967 Feb;118(2):448-55. doi: 10.1016/0003-9861(67)90373-6.
8
Effect of side-chain deuteration on protein stability.侧链氘化对蛋白质稳定性的影响。
Biochemistry. 1965 Jul;4(7):1213-25. doi: 10.1021/bi00883a002.
9
Electron micrographic investigations of C-phycocyanin.藻蓝蛋白的电子显微镜研究。
Arch Biochem Biophys. 1965 Jun;110(3):511-6. doi: 10.1016/0003-9861(65)90444-3.
10
Spectral change of phycoerythrin from hydrocoleum species and its relationship to protein dissociation. Effect of mercurials on single- and double-peaked forms.水生植物中藻红蛋白的光谱变化及其与蛋白质解离的关系。汞制剂对单峰和双峰形式的影响。
Biochim Biophys Acta. 1968 Feb 19;154(2):332-41. doi: 10.1016/0005-2795(68)90047-0.