藻胆蛋白的分离与光谱表征
Isolation and spectral characterization of phycobiliproteins.
作者信息
Teale F W, Dale R E
出版信息
Biochem J. 1970 Jan;116(2):161-9. doi: 10.1042/bj1160161.
Abstract
Several phycobiliproteins were prepared chromatographically pure and their absorption, fluorescence-emission, fluorescence-excitation and fluorescence-excitation polarization spectra determined. Changes in these spectra with ionic strength of the aqueous medium and chromoprotein concentration were interpreted in terms of interchromophore energy transfer and protein subunit equilibria. The complexity of the polarization spectra confirms the presence of different types of chromophore, designated sensitizing (;s') and fluorescing (;f'), in a single protein.
摘要
通过色谱法制备了几种纯的藻胆蛋白，并测定了它们的吸收光谱、荧光发射光谱、荧光激发光谱和荧光激发偏振光谱。根据发色团间的能量转移和蛋白质亚基平衡，解释了这些光谱随水介质离子强度和色素蛋白浓度的变化。偏振光谱的复杂性证实了在单一蛋白质中存在不同类型的发色团，分别称为敏化发色团（s）和荧光发色团（f）。
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本文引用的文献
1
POLARIZATION OF FLUORESCENCE OF MUSCLE PROTEINS.肌肉蛋白质荧光的偏振
J Biol Chem. 1965 Jul;240:2888-99.
2
FLUORESCENCE POLARISATION AUD LOCATION OF FLUORESCENCE MAXIMA OF C-PHYCOCYANIN.藻蓝蛋白荧光最大值的荧光偏振及定位
Biochim Biophys Acta. 1965 Mar 29;94:579-81. doi: 10.1016/0926-6585(65)90068-3.
3
C-PHYCOCYANIN: MINIMUM MOLECULAR WEIGHT.C-藻蓝蛋白：最小分子量
Science. 1964 Sep 4;145(3636):1054-6. doi: 10.1126/science.145.3636.1054.
4
Protein chromatography on calcium phosphate columns.在磷酸钙柱上进行蛋白质色谱分析。
Arch Biochem Biophys. 1956 Nov;65(1):132-55. doi: 10.1016/0003-9861(56)90183-7.
5
Energy transfer between chromophore and protein in phycocyanin.藻蓝蛋白中发色团与蛋白质之间的能量转移
Arch Biochem Biophys. 1954 Mar;49(1):222-33. doi: 10.1016/0003-9861(54)90182-4.
6
Structure of phycoerythrobilin and phycocyanobilin.藻红胆素和藻蓝胆素的结构。
Nature. 1967 Sep 30;215(5109):1477-8. doi: 10.1038/2151477a0.
7
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8
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9
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