Demidov A A, Mimuro M
Physics Department, Moscow State University, Russia.
Biophys J. 1995 Apr;68(4):1500-6. doi: 10.1016/S0006-3495(95)80322-X.
Absorption and fluorescence spectra of the C-phycocyanin beta-subunit were quantitatively deconvoluted into component spectra of the beta-84 and beta-155 chromophores. The deconvolution procedure was based on a theoretical treatment of polarization properties. Four kinds of spectra (absorption, emission, emission polarization, and excitation polarization) measured on C-phycocyanin isolated from the cyanobacterium Mastigocladus laminosus were used as the experimental data set. Without any assumption of spectral shape, the absorption and fluorescence spectra of both chromophores were unambiguously resolved and their fluorescence quantum yields were evaluated. By combining the spectra of the alpha-subunit, independently measured, with the resolved spectra of the beta-subunit, the fluorescence and fluorescence polarization spectra and the fluorescence quantum yield of the monomer were estimated; they agree with experimental values to within an acceptable error. Further, the matrix of energy transfer rates in the monomer was estimated; it gave a significantly different result (by up to 40%) from previously estimated ones.
藻蓝蛋白β亚基的吸收光谱和荧光光谱被定量解卷积为β-84和β-155发色团的组分光谱。解卷积过程基于对偏振特性的理论处理。从蓝细菌层理鞭枝藻中分离出的藻蓝蛋白上测得的四种光谱（吸收、发射、发射偏振和激发偏振）用作实验数据集。在没有任何光谱形状假设的情况下，明确解析了两种发色团的吸收光谱和荧光光谱，并评估了它们的荧光量子产率。通过将独立测量的α亚基光谱与解析后的β亚基光谱相结合，估算了单体的荧光光谱、荧光偏振光谱和荧光量子产率；它们与实验值在可接受的误差范围内相符。此外，估算了单体中的能量转移速率矩阵；它给出的结果与先前估算的结果有显著差异（高达40%）。
