Sebille B, Thuaud N, Tillement J P
J Chromatogr. 1979 Nov 28;180(1):103-10. doi: 10.1016/s0021-9673(00)80178-8.
A size exclusion chromatographic method for studying ligand-macromolecule binding parameters is described. This equilibrium saturation method allows the determination of the concentrations of constituents in equilibrium and is specially useful for characterizing ligand--protein binding under conditions that can be compared with physiological conditions. The method has been used for measuring warfarin--human serum albumin (HSA) binding and for studying the influence of free fatty acids (FFA) and sodium dodecyl sulphate on warfarin--HSA binding. Some comparisons with the Hummel and Dreyer method are given. The influence of the FFA is strongly dependent on their chain length, with an inversion of the effect for a 10-carbon chain.
描述了一种用于研究配体 - 大分子结合参数的尺寸排阻色谱法。这种平衡饱和法可测定平衡状态下各成分的浓度，特别适用于在可与生理条件相比较的条件下表征配体与蛋白质的结合。该方法已用于测定华法林与人血清白蛋白（HSA）的结合，并研究游离脂肪酸（FFA）和十二烷基硫酸钠对华法林 - HSA结合的影响。还给出了与Hummel和Dreyer方法的一些比较。FFA的影响强烈依赖于它们的链长，对于10碳链的情况，效果会发生反转。
