Sebille B, Thuaud N, Tillement J P
J Chromatogr. 1978 Dec 21;167:159-70. doi: 10.1016/s0021-9673(00)91155-5.
The binding to a biological macromolecule (human serum albumin, HSA) of small molecules (two drugs: warfarin and furosemide) has been studied by high-performance liquid chromatography. Two methods have been used and compared: frontal analysis and the Hummel and Dreyer methods. The association parameters of each of the two drugs on HSA were determined. The results obtained are in good agreement with those previously published using other techniques. The competition of these two drugs for the same site on HSA has also been shown.
通过高效液相色谱法研究了小分子（两种药物：华法林和呋塞米）与生物大分子（人血清白蛋白，HSA）的结合。使用并比较了两种方法：前沿分析法和胡梅尔-德雷尔法。测定了两种药物与HSA的缔合参数。所得结果与先前使用其他技术发表的结果高度一致。还展示了这两种药物在HSA上同一位点的竞争情况。
