Study of binding of low-molecular-weight ligand to biological macromolecules by high-performance liquid chromatography. Evaluation of binding parameters for two drugs bound to human serum albumin.

The binding to a biological macromolecule (human serum albumin, HSA) of small molecules (two drugs: warfarin and furosemide) has been studied by high-performance liquid chromatography. Two methods have been used and compared: frontal analysis and the Hummel and Dreyer methods. The association parameters of each of the two drugs on HSA were determined. The results obtained are in good agreement with those previously published using other techniques. The competition of these two drugs for the same site on HSA has also been shown.