委内瑞拉马脑炎病毒在人二倍体细胞株WI-38中的生长情况
Growth of Venezuelan equine encephalomyelitis virus in human diploid cell strain WI-38.
作者信息
Reitman M, Green L
出版信息
Appl Microbiol. 1970 Jan;19(1):196-8. doi: 10.1128/am.19.1.196-198.1970.
Abstract
We demonstrated that Venezuelan equine encephalomyelitis (VEE) virus replicated in and adapted rapidly to human diploid cell strain WI-38. Peak titers of approximately 10(9.8) mouse intracerebral 50% lethal doses were obtained at low passage levels in Eagles basal medium supplemented with calf serum. VEE virus replicated poorly in serum-free medium. Propagation of VEE virus was accompanied by the production of hemagglutinin and cytopathogenic effects.
摘要
我们证明委内瑞拉马脑炎（VEE）病毒可在人二倍体细胞株WI-38中复制并迅速适应该细胞。在补充有小牛血清的伊格尔基础培养基中低传代水平时，可获得约10(9.8)小鼠脑内半数致死剂量的峰值滴度。VEE病毒在无血清培养基中复制不佳。VEE病毒的增殖伴随着血凝素的产生和细胞病变效应。
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1
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Exp Cell Res. 1965 Mar;37:614-36. doi: 10.1016/0014-4827(65)90211-9.
3
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Proc Soc Exp Biol Med. 1965 Apr;118:1069-73. doi: 10.3181/00379727-118-30048.
4
THE IN VITRO GROWTH OF RUBELLA VIRUS IN HUMAN EMBRYO CELLS.风疹病毒在人胚胎细胞中的体外生长
Am J Epidemiol. 1965 Jan;81:71-85. doi: 10.1093/oxfordjournals.aje.a120499.
5
Application of a microtechnique to viral serological investigations.一种微技术在病毒血清学研究中的应用。
J Immunol. 1962 Mar;88:320-9.
6
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