Quinn P
J Exp Zool. 1979 Dec;210(3):497-505. doi: 10.1002/jez.1402100312.
When incubated for 8 to 26 hours with zona-free mouse or rat ova, human spermatozoa failed to attach to or penetrate any of the ova. The ova were capable of being fertilized since both intra- and inter-species penetration of spermatozoa and formation of pronuclei occurred between rat and mouse gametes. When mouse spermatozoa were incubated for three to eight hours with rat ova, a high proportion of the ova were penetrated, formation of pronuclei occurred and in 9 out of 36 ova incubated for 40 hours after insemination, regular cleavage and formation of morphologically normal 2-cell embryos occurred. Human spermatozoa retained their morphological integrity and motility only when the culture medium contained purified bovine serum albumin (3 mg/ml) or human serum (5% v/v) and not when unpurified BSA from several different commercial sources was used as a protein source. In this latter medium, the ova of both rats and mice degenerated after 8-hour incubation in the presence of human spermatozoa but not when human spermatozoa were absent or in the presence of either rat or mouse spermatozoa. Electron microscopy indicated that the human spermatozoa incubated for eight hours in medium containing purified BSA had undergone an acrosome reaction. These spermatozoa also attached to and penetrated human oocytes which had been matured in vitro.
当与去透明带的小鼠或大鼠卵子一起孵育8至26小时时，人类精子无法附着或穿透任何卵子。这些卵子能够受精，因为大鼠和小鼠配子之间发生了精子的种内和种间穿透以及原核的形成。当小鼠精子与大鼠卵子一起孵育三至八小时时，很大比例的卵子被穿透，出现原核形成，并且在授精后孵育40小时的36个卵子中有9个发生了正常分裂并形成了形态正常的2细胞胚胎。只有当培养基中含有纯化的牛血清白蛋白（3毫克/毫升）或人血清（5%体积/体积）时，人类精子才能保持其形态完整性和活力，而当使用几种不同商业来源的未纯化牛血清白蛋白作为蛋白质来源时则不能。在后者的培养基中，大鼠和小鼠的卵子在有人类精子存在的情况下孵育8小时后会退化，但在没有人类精子或有大鼠或小鼠精子存在时则不会。电子显微镜检查表明，在含有纯化牛血清白蛋白的培养基中孵育8小时的人类精子发生了顶体反应。这些精子还附着并穿透了在体外成熟的人类卵母细胞。
