Stanger J D, Quinn P
J Exp Zool. 1982 May 1;220(3):377-80. doi: 10.1002/jez.1402200313.
After a 1-hour preincubation of epididymal mouse spermatozoa at a concentration of 2 X 10(6)/ml in 0.23 mM spermine, the proportion of F1(C57BL X CBA) mouse ova fertilized after 1 and 2 hours was significantly greater than with untreated spermatozoa. Spermine also significantly increased the proportion of ova fertilized at the sub optimal sperm concentration of 2 X 10(5)/ml. The stimulatory effect was lost when the protein source in the fertilization medium was changed from human serum albumin V (HSA) to HSA crystalline. This provides indirect evidence that albumin is directly involved in the capacitation process and that the crystalline is more potent than the fraction V preparation. At equimolar concentrations, spermidine was partially and putrescine was totally inhibitory to fertilization. Mechanisms whereby spermine may affect metabolic activity or sperm-zona binding are discussed. It is suggested spermine may also be present in ovulatory fluid and therefore could potentially be involved in fertilization in vivo.
将附睾小鼠精子以2×10⁶/ml的浓度在0.23 mM精胺中预孵育1小时后，在1小时和2小时后受精的F1（C57BL×CBA）小鼠卵子的比例显著高于未处理的精子。精胺在精子浓度为2×10⁵/ml的次优条件下也显著提高了卵子受精的比例。当受精培养基中的蛋白质来源从人血清白蛋白V（HSA）变为HSA结晶时，刺激作用消失。这提供了间接证据，表明白蛋白直接参与获能过程，且结晶比V组分制剂更有效。在等摩尔浓度下，亚精胺有部分抑制作用，而腐胺对受精有完全抑制作用。文中讨论了精胺可能影响代谢活性或精子与透明带结合的机制。有人提出，精胺也可能存在于排卵液中，因此可能在体内受精过程中发挥作用。
