Orzelek R M, Wolen R L, Forney R B
J Forensic Sci. 1979 Jul;24(3):554-60.
Extravascular liver/blood and brain/blood ratios were found to be an average of 6% and 1% higher, respectively, in all experiments than total liver/blood and brain/blood ratios. This difference may be informative in establishing true tissue levels. There was a significant time effect (P less than 0.05) with the extravascular liver/blood ratios but not with the extravscular brain/blood ratios. Extravascular liver/blood ratios were slightly higher in phenobarbital-pretreated animals than in non-pretreated animals. Tissue secobarbital levels in pretreated and non-pretreated animals are not different at 1/4 or 1 h, even though pretreated animals received higher doses than non-pretreated animals. Tissue levels are significantly higher (P less than 0.01) in pretreated animals than in non-pretreated animals at 4 h. It is possible that, at this time period, the barbiturate-metabolizing enzymes have become saturated or exhausted.
在所有实验中，发现血管外肝脏/血液和大脑/血液的比率分别比总肝脏/血液和大脑/血液的比率平均高6%和1%。这种差异对于确定真实的组织水平可能具有参考价值。血管外肝脏/血液的比率存在显著的时间效应（P小于0.05），而血管外大脑/血液的比率则不存在这种效应。苯巴比妥预处理的动物的血管外肝脏/血液比率略高于未预处理的动物。尽管预处理的动物比未预处理的动物接受了更高的剂量，但在1/4小时或1小时时，预处理和未预处理动物的组织司可巴比妥水平并无差异。在4小时时，预处理动物的组织水平显著高于未预处理的动物（P小于0.01）。在这个时间段，巴比妥类代谢酶有可能已经饱和或耗尽。
