大鼠脑丙酮酸激酶：纯化及锂的作用

Rat-brain pyruvate kinase: purification and effects of lithium.

作者信息

Kajda P K, Birch N J, O'Brien M J, Hullin R P

出版信息

J Inorg Biochem. 1979 Dec;11(4):361-6. doi: 10.1016/s0162-0134(00)80191-9.

Abstract

Purified pyruvate kinase was prepared from pooled brains obtained from untreated rats. Its properties suggest that it is similar to type 'M' pyruvate kinase. Lithium inhibition was demonstrated at pharmacologically significant lithium concentrations (7%-12% at 2 mmol 1-1 Li) and this was similar in character to that previously seen in rabbit muscle pyruvate kinase, namely noncompetitive with respect to phosphoenol pyruvate, K+, and Mg2+ but competitive with ADP.

摘要

从未经处理的大鼠的汇集大脑中制备了纯化的丙酮酸激酶。其特性表明它类似于“M”型丙酮酸激酶。在具有药理学意义的锂浓度下（2 mmol/L Li时为7%-12%）证明了锂抑制作用，其性质与先前在兔肌肉丙酮酸激酶中观察到的相似，即对磷酸烯醇丙酮酸、K+和Mg2+无竞争性，但对ADP有竞争性。