Riggs B L, Bassingthwaighte J B, Jowsey J, Pequegnat E P
J Lab Clin Med. 1970 Mar;75(3):520-8.
A method is described for quantitating autoradiographs of bone-seeking isotopes in microscopic sections of bone. Autoradiographs of bone sections containing Ca and internal calibration standards are automatically scanned with a microdensitometer. The digitized optical density output is stored on magnetic tape and is converted by computer to equivalent activity of Ca per gram of bone. The computer determines the total Ca uptake in the bone section and, on the basis of optical density and anatomic position, quantitatively divides the uptake into 4 components, each representing a separate physiologic process (bone formation, secondary mineralization, diffuse long-term exchange, and surface short-term exchange). The method is also applicable for quantitative analysis of microradiographs of bone sections for mineral content and density.
本文描述了一种对骨切片中亲骨同位素的放射自显影片进行定量分析的方法。用显微密度计自动扫描含有钙和内校准标准物的骨切片放射自显影片。数字化的光密度输出存储在磁带上，并由计算机转换成每克骨中钙的等效活性。计算机确定骨切片中钙的总摄取量，并根据光密度和解剖位置，将摄取量定量地分为4个部分，每个部分代表一个单独的生理过程（骨形成、继发性矿化、弥漫性长期交换和表面短期交换）。该方法也适用于对骨切片微射线照片的矿物质含量和密度进行定量分析。
