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活细胞中膜脂相变的量热法检测

Calorimetric detection of a membrane-lipid phase transition in living cells.

作者信息

Reinert J C, Steim J M

出版信息

Science. 1970 Jun 26;168(3939):1580-2. doi: 10.1126/science.168.3939.1580.

DOI:10.1126/science.168.3939.1580
PMID:5420540
Abstract

The membrane lipids in living Mycoplasma laidlawii exhibit a phase transition characteristic of that from crystal to liquid crystal within the bilayer conformation. The transition occurs at the same temperature in viable organisms, membranes isolated from the organisms, and isolated membrane lipids. The enthalpy of the transition in the membrane is compared with that of an aqueous suspension of isolated membrane lipids. The result is consistent with presence of an extended lipid bilayer in the native membrane.

摘要

活的莱氏无胆甾原体中的膜脂在双层构象中呈现出从晶体到液晶的相变特征。在活的生物体、从生物体分离出的膜以及分离出的膜脂中,相变都在相同温度下发生。将膜中相变的焓与分离出的膜脂水悬浮液的焓进行比较。结果与天然膜中存在延伸的脂质双层一致。

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2
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Plant Physiol. 1980 Oct;66(4):596-9. doi: 10.1104/pp.66.4.596.
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Bull Math Biol. 1983;45(1):91-101. doi: 10.1007/BF02459389.
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