Anderson E L, Stephens J F
Appl Microbiol. 1970 May;19(5):726-30. doi: 10.1128/am.19.5.726-730.1970.
Studies were conducted to determine the effects of Salmonella anatum and S. heidelberg infections on the differential leukocyte counts of baby chickens. Newly hatched broiler-type chicks were inoculated in the yolk sac with suspensions of either S. anatum or S. heidelberg. At 0, 24, 48, and 72 hr after inoculation, blood was taken by heart puncture from chicks of both inoculated groups and from a group of uninoculated chicks. The averages of the leukocyte counts of three or four chicks from each group were used as the blood values for specific time intervals. The six classes of leukocytes counted were lymphocytes, monocytes, juveniles, heterophils, eosinophils, and basophils. The leukocytes classified as juveniles were immature or degenerate heterophils and were found almost exclusively in the infected chicks. Changes in heterophil, juvenile, and lymphocyte counts were affected by both the number of cells in the inoculum (300 versus 3 million cells of S. anatum) and species (S. anatum and S. heidelberg). Infection with either Salmonella species resulted in the development of a severe heterophilic leukopenia, and a significant increase in the percentage of both juveniles and lymphocytes by 48 hr postinoculation. Mortality rate was higher in groups of chicks inoculated with S. heidelberg than in groups given S. anatum.
开展了多项研究以确定鸭沙门氏菌和海德堡沙门氏菌感染对雏鸡白细胞分类计数的影响。刚孵出的肉用型雏鸡通过卵黄囊接种鸭沙门氏菌或海德堡沙门氏菌的悬液。接种后0、24、48和72小时，从两个接种组的雏鸡以及一组未接种的雏鸡中通过心脏穿刺采血。每组三四只雏鸡的白细胞计数平均值用作特定时间间隔的血液值。所计数的六类白细胞为淋巴细胞、单核细胞、幼粒细胞、异嗜性粒细胞、嗜酸性粒细胞和嗜碱性粒细胞。被归类为幼粒细胞的白细胞是未成熟或退化的异嗜性粒细胞，几乎只在受感染的雏鸡中发现。异嗜性粒细胞、幼粒细胞和淋巴细胞计数的变化受接种物中细胞数量（鸭沙门氏菌300个对300万个细胞）和菌种（鸭沙门氏菌和海德堡沙门氏菌）的影响。感染任何一种沙门氏菌都会导致严重的异嗜性白细胞减少，并且在接种后48小时幼粒细胞和淋巴细胞的百分比显著增加。接种海德堡沙门氏菌的雏鸡组死亡率高于接种鸭沙门氏菌的组。
