Nolte J, Brown J E
J Gen Physiol. 1970 Jun;55(6):787-801. doi: 10.1085/jgp.55.6.787.
Spectral sensitivity curves can be distorted by screening pigments. We have determined whether this is true for Limulus polyphemus by determining, from receptor potentials recorded using intracellular microelectrodes, spectral sensitivity curves for normal animals and for white-eyed animals (which lack screening pigment). Our results show: (a) In median ocelli, the curve for UV-sensitive receptor cells peaks at 360 nm and does not depend on the presence of screening pigment, (b) The curve for ventral eye photoreceptors is identical to that for retinular cells from the lateral eyes of white-eyed animals and peaks at 520-525 nm. (c) In normal lateral eyes, when the stimulating light passes through screening pigment, the curve indicates relatively more sensitivity in the red region of the spectrum than does the curve for white-eyed animals. Therefore, the screening pigment is probably red-transmitting, (d) In median ocelli, the curve for visible-sensitive cells peaks at 525 nm and is approximately the same whether the ocelli are from normal or white-eyed animals. However, the curve is significantly broader than that for ventral eyes and for lateral eyes from white-eyed animals.
光谱敏感性曲线会受到筛选色素的影响而发生畸变。我们通过使用细胞内微电极记录受体电位，分别测定正常动物和白眼动物（缺乏筛选色素）的光谱敏感性曲线，来确定对于美洲鲎而言情况是否如此。我们的结果表明：（a）在中眼，对紫外线敏感的受体细胞的曲线在360纳米处达到峰值，且不依赖于筛选色素的存在；（b）腹眼光感受器的曲线与白眼动物侧眼光感受细胞的曲线相同，在520 - 525纳米处达到峰值；（c）在正常侧眼中，当刺激光穿过筛选色素时该曲线表明，与白眼动物的曲线相比，光谱的红色区域相对更敏感。因此，筛选色素可能是透红色的；（d）在中眼，对可见光敏感的细胞的曲线在525纳米处达到峰值，无论这些中眼来自正常动物还是白眼动物，该曲线大致相同。然而，该曲线比腹眼和白眼动物侧眼的曲线明显更宽。
