Kreutzmann H, Fliedner T M
Scand J Haematol. 1979 Nov;23(5):360-6. doi: 10.1111/j.1600-0609.1979.tb02733.x.
The presence of CFU-C in the peripheral blood of 3 healthy volunteers was monitored over a period of 70 d. Blood samples were taken 3 times a week. Mononuclear cells (MNC) were separated from blood leucocytes by dextran sedimentation and the iron ingestion method. After cryopreservation the cells were cultured in soft agar. One sample of mononuclear cells was cultured using a leucocyte feeder layer as a source of colony stimulating activity (CSA) and another sample using human placental conditioned medium as CSA. In cultures using a leucocyte feeder layer, the average number of CFU-C was 29, 43, 118 per ml blood. No systematic oscillations were observed. When human placental conditioned medium was used the average number of CFU-C was 255, 125, 222 per ml blood, respectively. Significant oscillations with periods of 23, 19 and 25 d were observed.
在70天的时间里监测了3名健康志愿者外周血中集落形成单位 - 细胞（CFU - C）的存在情况。每周采集3次血样。通过葡聚糖沉降和铁摄取法从血液白细胞中分离出单核细胞（MNC）。细胞冷冻保存后，在软琼脂中培养。一份单核细胞样本使用白细胞饲养层作为集落刺激活性（CSA）来源进行培养，另一份样本使用人胎盘条件培养基作为CSA进行培养。在使用白细胞饲养层的培养中，每毫升血液中CFU - C的平均数量分别为29、43、118。未观察到系统性波动。当使用人胎盘条件培养基时，每毫升血液中CFU - C的平均数量分别为255、125、222。观察到周期为23、19和25天的显著波动。
