Harvey A R, Kaiser J, Clarke B J
Br J Haematol. 1984 Sep;58(1):129-36. doi: 10.1111/j.1365-2141.1984.tb06067.x.
The committed granulocyte-macrophage progenitor cell (or colony-forming unit, CFU-GM) differentiates in vitro under the influence of the soluble glycoprotein factor(s) termed colony stimulating activity (GM-CSA), which can be derived from several cellular sources, including normal human peripheral blood mononuclear cells (PBMNC). We have investigated the respective roles of the monocyte and T lymphocyte components of PBMNC in generating GM-CSA using as an assay CFU-GM colony formation by purified peripheral blood null cells. Highly purified unstimulated T-lymphocytes did not themselves stimulate null cell CFU-GM colony formation, but when monocytes were also included, large numbers of colonies were observed, far greater than those stimulated by monocytes alone. This synergism was independent of HLA phenotype, occurring with T-lymphocyte and monocyte fractions from unrelated donors. Media conditioned by unstimulated purified monocytes promoted CFU-GM colonies from null cells only in the presence of T-lymphocytes, suggesting that monocytes produce a factor directing the synthesis and/or release of GM-CSA by T cells.
定向粒细胞-巨噬细胞祖细胞（或集落形成单位，CFU-GM）在被称为集落刺激活性（GM-CSA）的可溶性糖蛋白因子影响下在体外分化，GM-CSA可来源于多种细胞，包括正常人外周血单个核细胞（PBMNC）。我们利用纯化的外周血裸细胞的CFU-GM集落形成作为检测方法，研究了PBMNC中的单核细胞和T淋巴细胞成分在产生GM-CSA中的各自作用。高度纯化的未刺激T淋巴细胞本身不会刺激裸细胞CFU-GM集落形成，但当同时加入单核细胞时，会观察到大量集落，远远多于仅由单核细胞刺激产生的集落。这种协同作用与HLA表型无关，在来自无关供体的T淋巴细胞和单核细胞组分之间也会发生。未刺激的纯化单核细胞条件培养基仅在有T淋巴细胞存在时才促进裸细胞形成CFU-GM集落，这表明单核细胞产生一种因子，指导T细胞合成和/或释放GM-CSA。
