[血液系统疾病中巨核细胞的细胞化学研究]

[Cytochemical studies in megakaryocytes in hematologic diseases].

作者信息

Kolarz G, Ottmeier H, Singer F, Pietschmann H

出版信息

Folia Haematol Int Mag Klin Morphol Blutforsch. 1975;102(5):507-14.

Abstract

Bone marrow smears of 48 patients consisting of 12 normal cases, 36 patients with different haematological diseases-among them 9 cases of idiopathic thrombopenia, 4 cases of polycythaemia, and 9 cases of Hodgkin's disease - were examined cytochemically. Acid phosphatase, unspecific esterases, naphthol-AS-D-chloroacetate esterase, peroxydase, and leucin-aminopeptidase were represented; in addition the PAS reaction, fastgreen staining at pH 1.1, methyl-green pyronin staining and the lipid representation with Sudan black B were carried out. Besides those responses known from literature the different behaviour of acid megacaryocyte phosphatase in different haematological diseases must be particularly emphasized from all reactions.

摘要

对48例患者的骨髓涂片进行了细胞化学检查，其中包括12例正常病例，36例患有不同血液系统疾病的患者——其中9例特发性血小板减少症，4例红细胞增多症，9例霍奇金病。检测了酸性磷酸酶、非特异性酯酶、萘酚-AS-D-氯乙酸酯酶、过氧化物酶和亮氨酸氨肽酶；此外，还进行了PAS反应、pH 1.1的固绿染色、甲基绿派洛宁染色以及用苏丹黑B进行脂质显示。除了文献中已知的那些反应外，在所有反应中，酸性巨核细胞磷酸酶在不同血液系统疾病中的不同表现必须特别强调。