Kruse H
Folia Haematol Int Mag Klin Morphol Blutforsch. 1975;102(4):389-402.
From 63 children with acute leukaemia the bone-marrow smears were cytochemically examined before the beginning of therapy. The activity of peroxydase was examined according to Sato and Sekya, that of acid phosphatase according to Löffler and Berghoff, that of alpha-naphthyl-acetate-esterase according to Gomori; the evidence of glycogen was examined by means of the PAS-diastase response according to McManus. Among the 63 cases of leukaemia we found 6 cases of paramyeloblastic leukaemia, 2 cases of parapromyelocytic leukaemia, and 3 cases of myelomonocytic leukaemia. 52 cases of leukaemia could not be further differentiated in morphological respect. They represented an immature paraleukoblastic leukaemia. A division according to leading cytochemical criteria was made for them. The therapeutic possibility of influencing the various groups was checked by means of prolonged observations. Children affected with paraleukoblastic leukaemia of the phosphatase type had a significantly low rate of remission similar to the myeloid leukaemia. Paraleukoblastic leukaemia of the PAS type, esterase type and the undifferentiated type revealed no essential differences. The rate of remission, however, was highest in leukaemia of the PAS type amounting to 100%. In one part of patients the prolonged cytochemical observations in 8 children with recidives showed that the cytochemical type under chemotherapy was changed.
对63例急性白血病患儿在治疗开始前进行了骨髓涂片的细胞化学检查。过氧化物酶活性按佐藤和关谷的方法检查，酸性磷酸酶活性按勒夫勒和贝霍夫的方法检查，α-萘乙酸酯酶活性按戈莫里的方法检查；糖原的检测采用麦克马纳斯的过碘酸-雪夫氏淀粉酶反应法。在63例白血病病例中，我们发现6例原粒细胞白血病、2例早幼粒细胞白血病和3例粒单核细胞白血病。52例白血病在形态学方面无法进一步区分，它们代表未成熟的原白血病细胞白血病，根据主要细胞化学标准对其进行了分类。通过长期观察来检验影响各个组别的治疗可能性。磷酸酶型原白血病细胞白血病患儿的缓解率明显较低，与髓性白血病相似。过碘酸-雪夫氏反应型、酯酶型和未分化型原白血病细胞白血病没有本质区别。然而，过碘酸-雪夫氏反应型白血病的缓解率最高，达100%。在一部分患者中，对8例复发患儿进行的长期细胞化学观察表明，化疗期间细胞化学类型发生了变化。
