Demchenko A P, Sandrovskiĭ A K
Ukr Biokhim Zh (1978). 1979 Nov-Dec;51(6):687-92.
The contents of tyrosine and tryptophan were determined by the method based on the first derivatives of the proteins absorption spectra within the alkali pH. The advantages of the method are as follows: a much better resolution of tyrosine and tryptophan spectral maxima located at 293 and 307 nm, respectively, which allows the precision of their assay to be increased, especially in case of a small amount of one amino acid and relative abundance of the other and high turbidity of the preparations; the account of the cystine absorption is not necessary; possibility to study proteins containing the chromophore prosthetic groups.
采用基于蛋白质在碱性pH值下吸收光谱一阶导数的方法测定酪氨酸和色氨酸的含量。该方法的优点如下：分别位于293nm和307nm处的酪氨酸和色氨酸光谱最大值分辨率更高，这使得它们的测定精度得以提高，特别是在一种氨基酸含量少而另一种氨基酸相对丰富以及制剂浊度高的情况下；无需考虑胱氨酸的吸收；能够研究含有发色辅基的蛋白质。
