Toner P G, Carr K E, Ferguson A, Mackay C
Gut. 1970 Jun;11(6):471-81. doi: 10.1136/gut.11.6.471.
The scanning electron microscope is capable of 25 mmu resolution combined with a great depth of focus, features which make the technique of value in the study of the intestinal mucosa. Surgical biopsies and postmortem specimens of small intestine have been examined using scanning and transmission electron microscopy. In biopsies, at low magnification, the villous pattern is seen while at higher magnification details of cell surfaces may be observed. Some features are best observed using scanning microscopy to examine dewaxed thick histological sections. In necropsy specimens villous architecture can still be satisfactorily assessed and details of the villous cores are seen, their collagen skeletons apparently less robust than the villi of the fresh biopsy. Scanning microscopy can extend the three-dimensional study of the small intestinal mucosa beyond the limits imposed by the resolution of the dissecting microscope.
扫描电子显微镜能够实现25毫微米的分辨率，并具有很大的焦深，这些特性使该技术在小肠黏膜研究中具有价值。已使用扫描电子显微镜和透射电子显微镜对手术活检组织和小肠的尸检标本进行了检查。在活检组织中，低倍镜下可见绒毛形态，高倍镜下可观察到细胞表面的细节。有些特征使用扫描显微镜检查脱蜡后的厚组织切片时能得到最佳观察效果。在尸检标本中，仍可令人满意地评估绒毛结构，并能看到绒毛核心的细节，其胶原骨架明显不如新鲜活检组织中的绒毛坚固。扫描显微镜可以将小肠黏膜的三维研究扩展到解剖显微镜分辨率所限制的范围之外。
