Borst-Pauwels G W, Goldstein D A
J Gen Physiol. 1970 Aug;56(2):180-90. doi: 10.1085/jgp.56.2.180.
Addition of a macromolecule to a solution will give rise to a large excluded volume for the centers of the solute molecules. This will cause an apparent increase in solute concentration which is of the same order of magnitude as that associated with the nonsolvent volumes reported in the literature. A critical examination of one of the procedures used for the determination of nonsolvent water-the vapor pressure method of Hill-is given, and it is concluded that, with the use of this method, it is impossible to detect any significant nonsolvent water surrounding bovine albumin for either sugars or polyols. Generally, data reported in the literature for the nonsolvent water of proteins or other macromolecules will be too high unless they are corrected for the excluded volume.
向溶液中添加大分子会导致溶质分子中心出现较大的排阻体积。这将导致溶质浓度明显增加，其数量级与文献中报道的非溶剂体积相关的浓度增加相同。对用于测定非溶剂水的一种方法——希尔的蒸气压法进行了批判性研究，得出的结论是，使用这种方法，无法检测到围绕牛血清白蛋白的任何显著量的非溶剂水，无论是对于糖类还是多元醇。一般来说，文献中报道的蛋白质或其他大分子的非溶剂水的数据会过高，除非对排阻体积进行校正。
