Ivancheva E, Russanov E
Acta Physiol Pharmacol Bulg. 1979;5(4):51-6.
The present work provided evidence that isolated rat liver mitochondria reduce the larger part of the added CU2+ and that the reduction is of important for copper accumulation in mitochondria. The process is rapid, energy-independent and affected by steric factors. Both mitochondrial thiols and cytochrome c could participate in the Cu2+-reduction. Blocking of these mitochondrial components decrease sharply but does not abolish the reduction ability of mitochondria. Copper ions undergoing reversible reduction and oxidation in the presence of cysteine and ferrocytochrome c lead to the formation of O2- and H2O2. Superoxide radicals arise only from reoxidation of Cu+.
目前的研究提供了证据，表明分离的大鼠肝脏线粒体可还原大部分添加的Cu2+，且这种还原对于线粒体中的铜积累至关重要。该过程迅速、不依赖能量且受空间因素影响。线粒体硫醇和细胞色素c均可参与Cu2+的还原。阻断这些线粒体成分会使还原能力大幅下降，但不会消除线粒体的还原能力。在半胱氨酸和亚铁细胞色素c存在的情况下，经历可逆还原和氧化的铜离子会导致O2-和H2O2的形成。超氧自由基仅由Cu+的再氧化产生。
