蛋白质与核酸乙酰化作用的比较。
Comparison of the acetylation of proteins and nucleic acids.
作者信息
Paik W K, Kim S
出版信息
Biochem J. 1970 Feb;116(4):611-5. doi: 10.1042/bj1160611.
Abstract
The possibility of acetylation of nucleic acids was examined. Although protein is actively acetylated with [1-(14)C]acetic acid in rat liver systems in vivo and in vitro and in a frog liver system in vivo, nucleic acids are not acetylated under these conditions; nucleic acids purified from these sources are without radioactivity. Requirements for acetylation in vitro of protein in rat liver are different from those in frog liver; GSH has no effect in the rat liver system and is inhibitory in the frog liver system. Among various acetylated proteins, proteins insoluble in 0.1m-sulphuric acid have the highest radioactivity.
摘要
对核酸乙酰化的可能性进行了研究。尽管在大鼠肝脏系统的体内和体外以及青蛙肝脏系统的体内，蛋白质能被[1-(14)C]乙酸有效地乙酰化，但在这些条件下核酸并未被乙酰化；从这些来源纯化的核酸没有放射性。大鼠肝脏中蛋白质体外乙酰化的条件与青蛙肝脏不同；谷胱甘肽在大鼠肝脏系统中没有作用，而在青蛙肝脏系统中具有抑制作用。在各种乙酰化蛋白质中，不溶于0.1m硫酸的蛋白质具有最高的放射性。
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Protein synthesis in isolated cell nuclei.分离细胞核中的蛋白质合成。
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