Parkman R, Levy J A, Ting R C
Science. 1970 Apr 17;168(3929):387-9. doi: 10.1126/science.168.3929.387.
Infection of mouse and rat cells by the murine sarcoma virus (Moloney isolate) showed two-hit kinetics for focus production in mouse cells but one-hit kinetics in rat cells. Antiserum added to cultures after infection suppressed focus formation in mouse cells but not in rat cells. These studies suggest that, in rat cells infected with murine sarcoma virus, leukemina virus is not needed for focus formation and that these foci result from proliferation of the transformed rat cell; in mouse cells, on the other hand, leukemia virus is needed as "helper," and focus formation requires spread of virus. The term "defectiveness" then, if used, should not be applied to RNA tumor viruses without qualification for the viral function studied and the cell system employed.
莫洛尼分离株鼠肉瘤病毒对小鼠和大鼠细胞的感染显示，在小鼠细胞中形成集落呈双打击动力学，而在大鼠细胞中呈单打击动力学。感染后加入培养物中的抗血清可抑制小鼠细胞中的集落形成，但不能抑制大鼠细胞中的集落形成。这些研究表明，在感染鼠肉瘤病毒的大鼠细胞中，形成集落不需要白血病病毒，这些集落是由转化的大鼠细胞增殖产生的；另一方面，在小鼠细胞中，白血病病毒作为“辅助病毒”是必需的，集落形成需要病毒传播。因此，如果使用“缺陷性”一词，在未对所研究的病毒功能和所采用的细胞系统进行限定的情况下，不应将其应用于RNA肿瘤病毒。
