鼠肉瘤病毒对小鼠3T3细胞的转化：在无复制性鼠白血病辅助病毒存在的情况下病毒样颗粒的释放

Bassin R H, Phillips L A, Kramer M J, Haapala D K, Peebles P T, Nomura S, Fischinger P J

Proc Natl Acad Sci U S A. 1971 Jul;68(7):1520-4. doi: 10.1073/pnas.68.7.1520.

Small numbers of virus-like particles were observed by electron microscopy in each of two cloned lines of 3T3 cells transformed by murine sarcoma virus, even though these lines were free of detectable quantities of infectious leukemia and sarcoma virus. The morphology and occurrence of the particles were identical to those of the murine leukemia-sarcoma group. Moreover, the particles incorporated uridine and had a buoyant density of 1.16 g/ml in sucrose gradients. No evidence of sarcoma or leukemia virus infectivity was associated with the particles in cells of several susceptible species under various conditions, including both cosedimentation with leukemia virus and infection in the presence of inactivated Sendai virus. The particles may represent a form of murine sarcoma virus deficient in one or more of the viral components necessary for infectivity.

在由鼠肉瘤病毒转化的3T3细胞的两个克隆系中，通过电子显微镜观察到少量病毒样颗粒，尽管这些细胞系中没有可检测到的传染性白血病和肉瘤病毒。这些颗粒的形态和出现情况与鼠白血病-肉瘤组的相同。此外，这些颗粒掺入了尿苷，在蔗糖梯度中的浮力密度为1.16克/毫升。在各种条件下，包括与白血病病毒共沉降以及在灭活仙台病毒存在下感染，在几种易感物种的细胞中，没有证据表明这些颗粒具有肉瘤或白血病病毒感染性。这些颗粒可能代表了一种在传染性所需的一种或多种病毒成分上有缺陷的鼠肉瘤病毒形式。