Charvat I, Esau K
J Cell Sci. 1975 Dec;19(3):543-61. doi: 10.1242/jcs.19.3.543.
The localization of acid phosphatase during xylem development has been examined in the bean, Phaseolus vulgaris. The azo dye, the final reaction product, is initially prominent in the dictyosomes, vesicles apparently participating in secondary wall formation, and in the middle lamella of the young vessel element. Final reaction particles are also present in mitochondria, chloroplasts, and certain vacuoles and are sparsely scattered in the cytoplasm. At a later stage of vessel differentiation, the azo dye is concentrated in the disintegrating cytoplasm and along the fibrils of the partially hydrolysed primary wall and middle lamella. In the mature vessel element, the azo dye is still present along the disintegrated primary wall at the side of the vessel and covers the secondary wall. In the parenchyma cell adjacent to the vessel element, acid phosphatase localization is found in the dictyosomes, endoplasmic reticulum, mitochondria, small vacuoles, and the middle lamella. The controls from all stages of vessel element development were free of azo dye particles. The concentration of acid phosphatase along the secondary walls of the mature vessels and in the middle lamella between other cells indicates that this enzyme has other functions besides autolysis of the cytoplasm and primary cell wall. Acid phosphatase may participate in the formation of the secondary wall and may also have a role in the secretion and transport of sugars.
在菜豆（Phaseolus vulgaris）中研究了木质部发育过程中酸性磷酸酶的定位。偶氮染料作为最终反应产物，最初在高尔基体、明显参与次生壁形成的小泡以及幼嫩导管分子的中胶层中很突出。最终反应颗粒也存在于线粒体、叶绿体和某些液泡中，并稀疏地散布在细胞质中。在导管分化的后期，偶氮染料集中在解体的细胞质中以及部分水解的初生壁和中胶层的纤维上。在成熟的导管分子中，偶氮染料仍沿着导管一侧解体的初生壁存在，并覆盖次生壁。在与导管分子相邻的薄壁细胞中，酸性磷酸酶定位于高尔基体、内质网、线粒体、小液泡和中胶层。导管分子发育各阶段的对照均无偶氮染料颗粒。成熟导管次生壁以及其他细胞之间中胶层中酸性磷酸酶的集中表明，这种酶除了参与细胞质和初生细胞壁的自溶作用外，还有其他功能。酸性磷酸酶可能参与次生壁的形成，也可能在糖类的分泌和运输中起作用。
