Vaughn K C, Duke S O
Histochemistry. 1981;72(2):191-8. doi: 10.1007/BF00517132.
NADH-dependent nitrate reductase (E.C. 1.6.6.1) was ultrastructurally localized in norflurazon-treated and control soybean cotyledons [Glycine max (L.) Merr.] by a method based upon the increase in osmiophilia due to the formation of an azo dye. The reaction product was observed in small vesicles throughout the cytoplasm. An apparent transport of nitrite to the plastid, the site of nitrite reduction, may occur through fusion of the nitrite-containing vesicles with the chloroplast envelope. Plants grown in tungstate lacked nitrate reductase activity as measured by standard assay procedures, and showed no increase in osmiophilia, suggesting a degree of specificity of this cytochemical procedure.
通过基于偶氮染料形成导致嗜锇性增加的方法，在超微结构水平上定位了NADH依赖性硝酸还原酶（E.C. 1.6.6.1）在去草净处理的和对照大豆子叶[大豆（Glycine max (L.) Merr.）]中的位置。在整个细胞质的小泡中观察到反应产物。含亚硝酸盐的小泡与叶绿体包膜融合，可能会出现亚硝酸盐向亚硝酸盐还原部位即质体的明显转运。用标准测定方法测量，在钨酸盐中生长的植物缺乏硝酸还原酶活性，且嗜锇性没有增加，这表明该细胞化学方法具有一定的特异性。
