Hebert G A
J Dent Res. 1976 Jan;55:A33-7. doi: 10.1177/002203457605500116011.
With all animal serums studied except rat serum, an improved salt fraction that contained a high percentage of gamma-globulin and little or no albumin could be obtained by using optimal ammonium sulfate concentrations. These concentrations were less than the routinely used half-saturated solutions and different from the sometimes quoted one-third-saturated solutions. These are simple, economical methods for obtaining the antibody-containing globulin fractions from the serums of a variety of animals commonly used as antibody producers for immunofluorescence applications. Fluorescent antibody reagents prepared in this laboratory from serum fractions obtained by these optimal procedures provided conjugates that may be superior to those prepared from fractions obtained with a higher and uniform salt concentration.
除大鼠血清外，对于所有研究的动物血清，通过使用最佳硫酸铵浓度可获得一种改良的盐级分，该级分含有高比例的γ-球蛋白且白蛋白含量很少或没有。这些浓度低于常规使用的半饱和溶液，且不同于有时引用的三分之一饱和溶液。这些是从常用于免疫荧光应用抗体产生的各种动物血清中获得含抗体球蛋白级分的简单、经济的方法。在本实验室中，由通过这些最佳程序获得的血清级分制备的荧光抗体试剂所提供的结合物可能优于由具有更高且均匀盐浓度获得的级分所制备的结合物。
