Hebert G A
Appl Microbiol. 1974 Feb;27(2):389-93. doi: 10.1128/am.27.2.389-393.1974.
Optimal (NH(4))(2)SO(4) concentrations were sought for serum fractionation in order to obtain the gamma globulin as free as possible from other serum components while maintaining a reasonable recovery. Various ammonium sulfate concentrations were used to fractionate sera from mice, hamsters, guinea pigs, monkeys, chimpanzees, swine, chicken, and cattle. All precipitates and supernatants were analyzed by electrophoresis to study the effects of various treatments on the composition of these materials. Approximately 75% of all the gamma globulins were recovered when each serum was fractionated with its optimal sulfate concentration. These optimals were determined to be as follows: three precipitations in 35% saturated ammonium sulfate (SAS) for hamster, chimpanzee, swine, and chicken serum; one precipitation in 35% SAS followed by two in 40% SAS for mouse and guinea pig serum; one precipitation in 30% SAS and then two in 40% SAS for monkey serum; and one precipitation in 30% SAS followed by two in 35% SAS for cattle serum.
为了在保持合理回收率的同时尽可能使γ球蛋白与其他血清成分分离，研究了用于血清分级分离的最佳硫酸铵浓度。使用不同浓度的硫酸铵对小鼠、仓鼠、豚鼠、猴子、黑猩猩、猪、鸡和牛的血清进行分级分离。通过电泳分析所有沉淀和上清液，以研究不同处理对这些物质组成的影响。当每种血清用其最佳硫酸盐浓度进行分级分离时，约75%的γ球蛋白得以回收。这些最佳浓度确定如下：仓鼠、黑猩猩、猪和鸡血清用35%饱和硫酸铵（SAS）进行三次沉淀；小鼠和豚鼠血清先用35% SAS进行一次沉淀，然后用40% SAS进行两次沉淀；猴子血清先用30% SAS进行一次沉淀，然后用40% SAS进行两次沉淀；牛血清先用30% SAS进行一次沉淀，然后用35% SAS进行两次沉淀。
