灌注大鼠心脏中氧化型谷胱甘肽和总谷胱甘肽的测定。
Measurement of oxidized glutathione and total glutathione in the perfused rat heart.
作者信息
Wendell P L
出版信息
Biochem J. 1970 May;117(4):661-5. doi: 10.1042/bj1170661.
Abstract
- A method was developed for the assay of GSSG in heart tissue. 2. GSSG and total glutathione were measured in rat hearts perfused under a variety of conditions. About 2% of the total glutathione is present as GSSG. The concentrations of GSSG and GSH remained constant under all the conditions tested. 3. These results are discussed with reference to the equilibrium and rate of the glutathione reductase reaction in the cell. It is concluded that the enzyme reaction does not lie near equilibrium.
摘要
- 开发了一种用于测定心脏组织中氧化型谷胱甘肽（GSSG）的方法。2. 在多种条件下灌注的大鼠心脏中测量了GSSG和总谷胱甘肽。总谷胱甘肽中约2%以GSSG的形式存在。在所有测试条件下，GSSG和还原型谷胱甘肽（GSH）的浓度保持恒定。3. 参照细胞中谷胱甘肽还原酶反应的平衡和速率对这些结果进行了讨论。得出的结论是，该酶反应并非处于接近平衡的状态。
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