Rauscher F M, Sanders R A, Watkins J B
Medical Sciences Program, Indiana University School of Medicine, Bloomington 47405-7005, USA.
J Biochem Mol Toxicol. 2000;14(6):329-34. doi: 10.1002/1099-0461(2000)14:6<329::AID-JBT5>3.0.CO;2-G.
Using diabetes mellitus as a model of oxidative damage, this study investigated whether subacute treatment (10 mg/kg/day, intraperitoneally for 14 days) with the compound piperine would protect against diabetes-induced oxidative stress in 30-day streptozotocin-induced diabetic Sprague-Dawley rats. Liver, kidney, brain, and heart were assayed for degree of lipid peroxidation, reduced and oxidized glutathione (GSH and GSSG, respectively) content, and activities of the free-radical detoxifying enzymes catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase. Piperine treatment of normal rats enhanced hepatic GSSG concentration by 100% and decreased renal GSH concentration by 35% and renal glutathione reductase activity by 25% when compared to normal controls. All tissues from diabetic animals exhibited disturbances in antioxidant defense when compared with normal controls. Treatment with piperine reversed the diabetic effects on GSSG concentration in brain, on renal glutathione peroxidase and superoxide dismutase activities, and on cardiac glutathione reductase activity and lipid peroxidation. Piperine treatment did not reverse the effects of diabetes on hepatic GSH concentrations, lipid peroxidation, or glutathione peroxidase or catalase activities; on renal superoxide dismutase activity; or on cardiac glutathione peroxidase or catalase activities. These data indicate that subacute treatment with piperine for 14 days is only partially effective as an antioxidant therapy in diabetes.
本研究以糖尿病作为氧化损伤模型，探讨了用胡椒碱化合物进行亚急性治疗（10毫克/千克/天，腹腔注射14天）是否能预防30天链脲佐菌素诱导的糖尿病Sprague-Dawley大鼠的糖尿病诱导氧化应激。对肝脏、肾脏、大脑和心脏进行脂质过氧化程度、还原型和氧化型谷胱甘肽（分别为GSH和GSSG）含量以及自由基解毒酶过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶和谷胱甘肽还原酶活性的检测。与正常对照组相比，胡椒碱处理正常大鼠可使肝脏GSSG浓度提高100%，使肾脏GSH浓度降低35%，肾脏谷胱甘肽还原酶活性降低25%。与正常对照组相比，糖尿病动物的所有组织在抗氧化防御方面均表现出紊乱。胡椒碱处理可逆转糖尿病对大脑中GSSG浓度、肾脏谷胱甘肽过氧化物酶和超氧化物歧化酶活性以及心脏谷胱甘肽还原酶活性和脂质过氧化的影响。胡椒碱处理并未逆转糖尿病对肝脏GSH浓度、脂质过氧化、谷胱甘肽过氧化物酶或过氧化氢酶活性、肾脏超氧化物歧化酶活性或心脏谷胱甘肽过氧化物酶或过氧化氢酶活性的影响。这些数据表明，胡椒碱14天的亚急性治疗作为糖尿病的抗氧化疗法仅部分有效。
